Student Interest Groups (SIG) project

Supervisor: Professor Tsia, Kevin K.M. (Department of Electrical and Electronic Engineering)

Project information

Project descriptions

Antimicrobial resistance is a global phenomenon arising from the indiscriminate use of antibiotics. To combat this problem, the Antimicrobial Peptide Cecropin B (CecB), a highly-effective, broad-spectrum antibiotic alternative is expressed in transgenic Chlorella autotrophica, a well-studied and highly-productive protein expressing microalgal chassis. It is expressed with a secretion signal peptide for extraction; and SUMO-tag for in-vitro cleavage using SUMO-protease. To validate efficacy, the isolated CecB is tested against common aquatic pathogens. The in-vitro cleavage system, barring the signal peptide, is also expressed using a bacterial chassis. This is to test the design and quantify the differences between microalgal and bacterial synthetic capabilities. To modify the system for industrial use, the signal peptide is removed and the SUMO-protease is additionally inserted, under a nitrogen-starvation inducible promoter, to cleave SUMO in-vivo and generate active AMPs. The microalgae are then freeze-dried, preserving active AMPs internally, which can then be used as supplemental feed.

Team information

Project leader: Vicera Christian Nelson Tejedor, BSc

Team member(s): Angel Ng, BEng; Sathya Balaji, BASc; Jegadishwar Ananda, BSc; Clara Pun, BSc; Louis Yu, BSc; Angela Fan, BSc; Eugenia Fong, BSc; Sneha Balasubramanian, BBiomedSc; Tanvi Falodiya, BBiomedSc; Sarah Khan, BSc; FeiFei Long, BSc; Rita Lim, BSc; Vismaya Pillai, BSc; Sunanas Kazi, BEd&BSc

Project poster
Project video
Project images

Winner of the Inno Show Award @ The 6th Inno Show

This project team was selected for the Inno Show award at the 6th Inno Show. 

This team has received HK$20,000 sponsorship to participate in international design competition(s) and consumables expenses to support further development of the project.

The best project award - COMP3329 Computer Game Design and Programming @ The 1st Engineering InnoShow

This project team was selected for the best project award – COMP3329 Computer Game Design and Programming at the 1st Engineering InnoShow.

Gold Medal at the 2022 iGEM Giant Jamboree

This project team was awarded for the Gold Medal at the 2022 iGEM Giant Jamboree.

PERfECT Wearables: for Minimally-Invasive and Continuous Blood-Glucose Monitoring was selected for the Inno Show Award
Photos in the Engineering Inno Show