Single cell traction force measurement assay with multiphoton-based protein micropatterns

A multiphoton-based protein micropatterning technique was developed in our lab previously. In this project, protein micropillar arrays were fabricated for cell seeding, and it was utilized for single cell traction force measurement. On the other hand, a method for encapsulating cells in collagen gel for cell therapy was also developed in the lab before. In order to improve the efficiency of cell therapy using this method, characteristics of these cells has to be identified. Therefore, the traction force of stem cells that are migrated out from the gel and the cells that are not encapsulated in the gel were measured and compared to understand the differences between them.